FastQCFastQC Report
Thu 26 May 2016
SRR1511913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381266
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25500.18461324610900434No Hit
TATCAACGCAGAGTACTTTTTTTTT14930.10808924566303667No Hit
GGTATCAACGCAGAGTACTTTTTTT14840.107437669500299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.013.1699711
AACACCG701.0869902E-412.21878055
GTCCTAA2650.011.8797351
GCATAAG907.119619E-611.659741
TCCAACG907.47334E-611.60907518
GTCTAAC751.9945316E-411.4477451
CTAACCT951.3542443E-511.0040484
GTATCAA12450.010.9573471
GTAATAC700.001486649210.8611383
CTTAGAC1408.296411E-910.8611383
GTCCTAC5450.010.8526021
GTATAGG2200.010.8406691
ATTACAC1502.1173037E-910.7706293
TTAGGCT1158.7702574E-710.7430834
CGAAATC803.786334E-410.68253113
TAATACT1355.6521458E-810.5594414
TTAGACT909.476971E-510.559444
GGACCGT1002.3996066E-510.4493036
TAGGACC2650.010.4000534
ATTTCTA3400.010.3334315
Produced by FastQC (version 0.11.5)