FastQCFastQC Report
Thu 26 May 2016
SRR1511912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305982
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18320.1402775842239786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG250.006035646418.997784
ACTATAC851.7183993E-814.5277153
TAAAACG400.00528260214.2483344
CGTGCGC655.4571698E-513.15230810
GGTATCA5900.012.4106361
GTCTTAG1251.3878889E-912.1720931
GTACTAG1052.683537E-711.7736021
GGACCGC658.029787E-411.6909416
CCTGCTA600.00587992211.0820388
TAGTACT951.3613861E-510.99871354
GTATCAA13150.010.991911
GCATTAA803.7353332E-410.6981291
GTCCTAA1105.970409E-610.3739441
GATCTAA1105.970409E-610.3739441
GTATTAG1303.7858263E-710.2409441
TACTATA1151.0136882E-59.9229022
GTGTATA1553.995956E-89.8162051
AGTAGAG1554.0652594E-89.8053065
TAATACT2054.1836756E-119.7305714
GCATAAT1002.7267175E-49.5094491
Produced by FastQC (version 0.11.5)