FastQCFastQC Report
Thu 26 May 2016
SRR1511912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305982
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20650.15811856518696277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG500.001496341713.3035134
GGCTCGA550.003081314312.08391811
CCCTATA951.0360254E-612.0031712
GCCTCGA1052.7347778E-711.75645316
TCGAACT907.4645886E-611.61017419
GGCGTGC907.4996788E-611.6052858
GTCTAGA1604.1836756E-1111.3346121
GGTATCA5600.011.2493891
GTATCAA12850.011.21622851
GATCTAA600.00567198511.1357591
ATTACAC1408.307325E-910.8600113
CTCTTCG700.001496904110.8521113
GGGTAAG1553.3833203E-910.468641
CTCGAAC1002.3997012E-510.44915718
GTATTAA1752.1645974E-1010.3630731
CCTTATA1201.5173682E-610.2943852
GTATAAA2053.6379788E-1210.2433461
CTAGAAC1502.431625E-810.136013
GTCTAGC856.3115073E-410.1064041
AGCGCAG856.626584E-410.05300710
Produced by FastQC (version 0.11.5)