FastQCFastQC Report
Thu 26 May 2016
SRR1511911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118698
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18380.1642981394442468No Hit
GGTATCAACGCAGAGTACTTTTTTT11590.10360258085738956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAC707.1774484E-613.5861321
GGTATCA5200.012.8023171
ATTAGAC905.408601E-712.6650873
TAGGCCC853.946021E-612.2925844
TGGTATC1302.568413E-911.7044522
GCCTTAT752.0520786E-411.4123511
ATAATAC1303.266905E-810.9601713
ACCGTGC700.001492387510.855798
GTTTTAG1158.684274E-710.7507661
AAGGCGT1106.0481507E-610.3623456
GTCTAGA1201.5007881E-610.3028171
CTGTTAG1201.5007881E-610.3028171
CTAGAAC1409.689393E-810.1773033
GTATTAA1803.8562575E-1010.0386421
GATTAAG1054.0528947E-59.9631631
TGTAGGG1252.5327608E-69.8902622
GTGTAGG1553.9875886E-89.8170761
GTGGTAT1651.0017175E-89.7984831
GTATCAA10150.09.6508391
GTTCTAA800.00447363369.5102921
Produced by FastQC (version 0.11.5)