FastQCFastQC Report
Thu 26 May 2016
SRR1511911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118698
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19570.1749355053821496No Hit
GGTATCAACGCAGAGTACTTTTTTT11730.10485403567361343No Hit
TATCAACGCAGAGTACTTTTTTTTT11400.10190417789251434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG250.006018834719.0066665
GACCGTC551.9699286E-413.8094377
GGTATCA5750.012.7851241
AAGGCGT951.0389831E-611.9999166
GTCTTAG1950.011.7505631
TGGACCG657.996756E-411.6964115
GCACGGC658.0569915E-411.68595413
GGCGTGC752.0850475E-411.3927858
AGAGTCG600.005858591311.0872225
TGGATCG600.005858591311.0872225
TCGTGGC600.005901655611.076329
CACGCCA700.001486477910.8609524
AGGCGTG1053.4891364E-610.8502727
CCTATAC909.474909E-510.5592593
CCTACAC1303.8172766E-710.234363
ATAGGAC750.002638886410.13688853
GTTAGAC856.5686397E-410.0623533
GCAATAC856.5686397E-410.0623533
GTCATAA1053.8833627E-510.0019671
GTACCAA1053.8833627E-510.0019671
Produced by FastQC (version 0.11.5)