FastQCFastQC Report
Thu 26 May 2016
SRR1511910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358012
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21840.16082332114885584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTC551.9616063E-413.81673511
GGTATCA6800.012.8643851
AAGACCG752.0754636E-411.3988085
AGGCGTG1104.972535E-711.2260977
TGGACCG951.3613117E-510.9988495
CCCTTAA1158.7110857E-710.74869251
GTATTGG1105.9784015E-610.3728631
GAATAAG1752.3101165E-1010.3234681
TAATACA1752.3646862E-1010.3132064
ACTTAGT750.002649502110.1322748
AGTCGTC750.002649502110.1322748
GTGGTAT2850.010.00890351
TCCAACG951.6452123E-49.99895418
ATTGCAC951.6452123E-49.9989543
TCTAATC1054.063238E-59.9612422
GGCGAGG2400.09.89479719
GTCCTAG1451.5946353E-79.8363361
CTAACAC1554.0650775E-89.8054253
GTAGGAC5250.09.5894733
GGTTTAG900.00110429589.5084591
Produced by FastQC (version 0.11.5)