FastQCFastQC Report
Thu 26 May 2016
SRR1511910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358012
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23780.17510890919962416No Hit
GGTATCAACGCAGAGTACTTTTTTT14580.1073628215361867No Hit
TATCAACGCAGAGTACTTTTTTTTT14450.10640553986268161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.013.6477821
CAGTTCG550.003077872312.0857789
CTAGCAC1052.7141868E-711.763413
ACTATCC907.493836E-611.6061838
CTAGACT1001.9258296E-611.4014594
GTCTAAA1451.1350494E-911.1842671
ATTACAC1605.4205884E-1010.6888673
ACGAAAT803.7848303E-410.68296312
TTAGACA1252.2345012E-710.6413614
TCCAACG1252.2401764E-710.63940118
GTACTAG1002.2903569E-510.4934731
GTAGAAC3900.010.4756983
CCAACGA1002.3992483E-510.44941219
CCGTTTC1002.4080406E-510.44594913
TAGAACA4350.010.0472624
TAGGACC2850.010.001284
CTAAGCT951.6413545E-410.0012794
CTTAGAC951.6413545E-410.0012793
CTCTTAT1053.920155E-59.9937841
GTATCAA14450.09.9686351
Produced by FastQC (version 0.11.5)