FastQCFastQC Report
Thu 26 May 2016
SRR1511909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311553
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23150.17650830732726777No Hit
TATCAACGCAGAGTACTTTTTTTTT14380.10964101336354687No Hit
GGTATCAACGCAGAGTACTTTTTTT14130.10773487613539065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.012.6822421
TTTGGAC752.0761281E-411.3983543
TGGCGAG4250.011.39835318
CTTAGAC1104.9746086E-711.225653
GTCCTAA2900.011.1516271
CAATACC1158.827046E-710.7375794
TCTATAC1158.827046E-710.7375793
GTAAGAC803.774866E-410.6859553
TAGGACC1701.382432E-1010.6161144
TTAGACC909.525502E-510.554034
GTATAAG1451.4142643E-810.4956491
GCGTGCG1002.4014154E-510.448499
GGCGAGA2950.010.30359619
ATGGCGA4550.010.22929217
TATGGCG4650.010.21357716
TACCCTT750.002650274210.1318695
AGGACCG750.002650274210.1318695
CGCCTGA750.002650274210.13186919
GTCCCGC750.002650274210.13186912
GTAGGAC4650.010.0093063
Produced by FastQC (version 0.11.5)