FastQCFastQC Report
Thu 26 May 2016
SRR1511909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311553
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23370.17818570808804524No Hit
GGTATCAACGCAGAGTACTTTTTTT15170.1156644070045206No Hit
TATCAACGCAGAGTACTTTTTTTTT14820.11299581488510187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.012.7247021
GTCCTAA2450.011.6859511
GTCTTAC907.0878505E-611.66430951
ATAGTAC1001.9228173E-611.4028623
GTCCTAC4350.011.1889621
ATTTAGA2100.010.9068871
GTCTTAA2050.010.7073711
GCGTGCG803.7863618E-410.6824399
TCCTACA5100.010.6203122
GTCTAAA1355.3129042E-810.6039191
ACCGTGC1002.4101535E-510.4450518
TAGGACC2400.010.29425054
CCTATAC1303.824698E-710.2333363
GTTCTAA1909.094947E-1110.0458171
GATATAC951.569273E-410.0458171
TAATACT2400.09.8983174
TGGCGAG3950.09.8598518
TCTATAC1651.0162694E-89.7903363
GTATCAA12250.09.6603861
TAGACAG1606.600203E-89.5023855
Produced by FastQC (version 0.11.5)