FastQCFastQC Report
Thu 26 May 2016
SRR1511908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649103
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG400.005242609414.264981
CCGTATG500.001501113313.2982389
TGTGCGG701.0923286E-412.21266710
ACCGTAT701.0923286E-412.2126678
TAGGACC2300.011.9766744
GTTCTAA1052.6832276E-711.7742691
GGTATCA5600.011.378021
TTAGGAC4400.011.2257853
TCTGTAC2200.010.7940243
GTATCAA12150.010.7231951
AGGACGT7850.010.5272695
TCTAGAC1909.094947E-1210.498613
CATCTAG1451.4195393E-810.4937791
TAGGACG7750.010.4179744
GGACGTG7600.010.2486436
CTAGGAC2350.010.1050443
GTAGGAC7300.09.8891013
GCGCCAC1252.5737081E-69.87869213
GTATTAC1356.3489097E-79.8622091
GTATAAC1356.3489097E-79.8622091
Produced by FastQC (version 0.11.5)