FastQCFastQC Report
Thu 26 May 2016
SRR1511908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649103
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17790.10787682758445045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT500.001496135513.3041014
CCCGGAC604.081633E-412.6705713
TAGACTG1950.011.6959115
GTCCTAC7350.011.5570991
AGGACCG752.0680322E-411.4035145
GGTATCA6900.011.2042321
GTATAAT2450.010.90782451
CTACACG1053.4532477E-610.860494
GTCCTAG2550.010.8543541
CTAAATT5350.010.8311719
GTATCAA13900.010.6429761
GTCCTAT909.076035E-510.6048291
TAGGACC2700.010.5588094
CTTAGAC1201.5174228E-610.2948393
TAGTACC1303.8242797E-710.2339224
TCCAACG1409.6933945E-810.17800218
TGCACCG750.00264095610.13645655
TCCTACA8450.010.1214632
TAAGCTG1054.0907413E-59.9554495
GTCCTAA3750.09.926121
Produced by FastQC (version 0.11.5)