FastQCFastQC Report
Thu 26 May 2016
SRR1511907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847060
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28570.33728425377186977No Hit
GGTATCAACGCAGAGTACTTTTTTT16620.19620806082213776No Hit
TATCAACGCAGAGTACTTTTTTTTT14610.17247892711260124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAACG350.002153840416.3029382
GTCTAGA957.269955E-813.0137481
GGTATCA6150.012.9893321
TAGGACC1501.8189894E-1212.6650924
AGGACCG550.003069321212.0894065
GTGGTAT2050.012.06152251
GTATTAG657.9460256E-411.7046731
TCTAGGG752.0740846E-411.3985833
GTATTAC855.259057E-511.1882911
GTATTAA1355.5824785E-810.5667191
GTATGAA1355.5824785E-810.5667191
GAGTATA1002.3670571E-510.4610521
CTTACAC1106.0425173E-610.3623483
GTAGGAC2300.010.3248033
ATTGGAC856.593877E-410.0575733
GTATTGG1054.0508843E-59.9629061
GGACGTG1554.0563464E-89.8052326
TTAGGAC1651.0199074E-89.7866623
GTATCAA11600.09.7559961
GTCCTAG1304.171796E-69.5100471
Produced by FastQC (version 0.11.5)