FastQCFastQC Report
Thu 26 May 2016
SRR1511907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847060
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33960.4009161098387363No Hit
GGTATCAACGCAGAGTACTTTTTTT19120.22572190871957123No Hit
TATCAACGCAGAGTACTTTTTTTTT15860.187235851061318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.013.519341
GTCCTAG802.7212296E-511.9339531
GTCTAAG802.7212296E-511.9339531
GGCTTAT600.00565934211.1383561
TGGTATC1803.6379788E-1211.0883312
GTATCAA14500.010.798171
GTTTTAG1252.0867083E-710.69282151
TAGGGAG1355.6323188E-810.5603165
GTGGTAT2100.010.4564161
TAGGACG1106.0011607E-610.368314
GGACGTG1201.520566E-610.2914436
TTAGGTG750.002635564210.1379035
TAAGGTT750.002635564210.1379034
CTTAGTC750.002635564210.1379033
GTTTTAC856.29309E-410.108761
GTATTAT1159.657941E-69.9622561
CTCTTAT1502.456327E-79.5471631
GTACTAC900.0010631299.5471631
GTATTAA1002.617952E-49.5471621
GTGTAAG1909.567884E-109.5471621
Produced by FastQC (version 0.11.5)