FastQCFastQC Report
Thu 26 May 2016
SRR1511906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223752
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30440.24874320940844225No Hit
TATCAACGCAGAGTACTTTTTTTTT18770.15338075034810975No Hit
GGTATCAACGCAGAGTACTTTTTTT17910.1463531826709987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1900.012.9985474
GGTATCA6350.012.5788861
CTTACGG550.003070374712.0895589
GGTCTAT802.83744E-511.8862991
CCCCTAC752.0548938E-411.4108471
GGCGAGG2200.011.22601819
TAATACC855.3265456E-511.1752214
ATGCCGT600.00587934211.0820936
TCAATAC700.001492401410.8559283
GTCATAA909.419091E-510.56559851
GTGGTAT2250.010.56559851
TAGGTTG1002.4001893E-510.4488325
TGGCGAG5100.010.43020618
GTCCTAA2950.010.314891
TACACAG4350.010.263225
CTACACA3350.010.2078134
GTATCAA12900.010.0250341
TCAAGAC951.6450301E-49.9988823
GAGTAGG1054.059619E-59.961851
GTAGGAC6050.09.8914573
Produced by FastQC (version 0.11.5)