FastQCFastQC Report
Thu 26 May 2016
SRR1511906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223752
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32450.2651681059561088No Hit
GGTATCAACGCAGAGTACTTTTTTT19270.15746654550922085No Hit
TATCAACGCAGAGTACTTTTTTTTT18940.15476992070288753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG456.7930395E-414.7685297
CTAGTAC602.5528792E-514.258563
GCCGGTT602.5835749E-514.240511
GGTATCA6350.013.8325981
CAAGACT1102.7193892E-912.9623274
CGGTTTC701.096926E-412.20664213
CCGGTTT701.0973444E-412.20614312
ACTGCGT658.063513E-411.6849918
TAGGACG6100.011.2198514
TAACCTG1201.26347E-711.0899925
TCCAACG1201.2785677E-711.08047218
TGCCGGT951.3695124E-510.99266810
GGCGAGG3200.010.9815419
GTATTAC1053.2626613E-610.9114281
TAGTACT1053.4388013E-610.8636654
GGACGTG6150.010.8141956
AGGACGT6100.010.7523575
GACGTGA3800.010.7432737
GTCCTAA3400.010.6707351
GTATCAA14200.010.4215671
Produced by FastQC (version 0.11.5)