FastQCFastQC Report
Thu 26 May 2016
SRR1511905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430235
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19950.13948756672854462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.013.0432841
TAGGACC2850.012.3317224
TCTACAC2650.011.4701993
ACCGTAT600.00588098711.0818858
TTTTAGA1303.2119715E-810.9735342
TTGGACA2000.010.9235724
TAGACTG1951.8189894E-1210.7165485
GTAGGAC5250.010.6747953
GTCTTAC1355.5901182E-810.5674761
CTTACAC2250.010.5541763
TACTATA1002.369233E-510.4614342
GTCCTAA2200.010.375341
GTCTAGG1409.5225914E-810.1900661
TGTAGGA5150.010.15673352
TTAACCC750.00265022110.13200954
TATTACA1701.5279511E-910.0698312
TGCACCG856.6001806E-410.0575095
GACTAGA951.624998E-410.0112931
AGGACCG1451.6188278E-79.8263035
ATAGAAC1651.0231815E-89.78663
Produced by FastQC (version 0.11.5)