FastQCFastQC Report
Thu 26 May 2016
SRR1511905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430235
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20200.1412355312238898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA508.3861814E-515.2683031
TCCAACG655.4556604E-513.15287818
GGTATCA5350.012.3073941
GTTCTAG1302.4356268E-911.7448491
GTATCAA12500.011.2222041
GGCGTGT600.005898412311.077488
GTACTAG951.2939281E-511.049431
GCCTAGA951.2939281E-511.049431
TAGAACA2750.010.7127994
TGATACT1252.2281529E-710.6436834
ATAATAC1355.6546924E-810.559213
AAGGCGT1002.3999539E-510.449236
GTGTTAG1658.58563E-1010.4102071
CAGGACT3400.010.3418154
GTCTTAC1201.4377474E-610.3379141
TTAGGAC2350.010.1098813
TGCACCG951.6378815E-410.0034635
TAGGACC2201.8189894E-129.9352574
TAGGACT2300.09.9164764
AGGACTG4050.09.8552635
Produced by FastQC (version 0.11.5)