FastQCFastQC Report
Thu 26 May 2016
SRR1511904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085771
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21280.19598976211374222No Hit
TATCAACGCAGAGTACTTTTTTTTT13370.12313830448593673No Hit
GGTATCAACGCAGAGTACTTTTTTT13220.12175679770411994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA500.001500464613.2983245
GGTATCA5450.012.7378321
TTATACT1451.2169039E-911.1365274
CGTCAGG700.001478068810.8682871
GTAATAC700.001492343110.8557753
GTATTAG803.7331376E-410.698471
GTGGTAT2050.010.66947651
GTCTTAG1252.2052518E-710.6509221
TAGCACT1659.295036E-1010.362334
GAATTAG856.5276644E-410.0691481
TTAGGAC2002.5465852E-119.97374253
GTATCAA11750.09.9548881
CTCCTAG1151.0127993E-59.9232181
ATTATAC1252.5704758E-69.8787563
TGGTATC2151.0913936E-119.7304592
GGATATA800.0044755579.5097511
CCTCTAA900.00110254839.5097511
TCTAATA1201.6787515E-59.5093132
GCTACAC1804.1782187E-99.4988033
TAGGACG2800.09.4988034
Produced by FastQC (version 0.11.5)