FastQCFastQC Report
Thu 26 May 2016
SRR1511904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085771
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24920.22951432668582972No Hit
GGTATCAACGCAGAGTACTTTTTTT14940.13759807546895247No Hit
TATCAACGCAGAGTACTTTTTTTTT14810.13640076959137792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCG456.740139E-414.7835145
GGTATCA5050.013.7945961
ACACCGC500.001499340313.2996456
CTTATAC801.9891286E-613.0675713
GTATTAG701.04546E-412.2693621
GATCTAT550.002965759512.145431
CTAGACA951.0339427E-612.0046584
GAGTATA951.2929071E-511.0496011
GTCCTAG1407.768904E-910.90611
GCCTATA803.6151864E-410.7356921
GTATCAA12000.010.6561691
CCCTAGA909.084003E-510.6031531
TAATTCC909.470743E-510.5596534
TAAGGAG1355.6448698E-810.5596535
GTCCTAT1105.7228644E-610.4103681
GTATAGT1701.4388206E-910.1041811
ACGTGAA2107.2759576E-129.9470748
ACTGACC1151.031745E-59.9077578
TTAGGAC2400.09.8996743
TGGCGAG2400.09.89465818
Produced by FastQC (version 0.11.5)