FastQCFastQC Report
Thu 26 May 2016
SRR1511903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27470.17638054536068443No Hit
GTCCTAAAGTGTGTATTTCTCATTT23870.15326551211356157No Hit
CTGTAGGACGTGGAATATGGCAAGA23190.14889933916688283No Hit
GTATCAACGCAGAGTACTTTTTTTT21270.136571321435084No Hit
CTTTAGGACGTGAAATATGGCGAGG20510.13169148108291362No Hit
GTCCTACAGTGTGCATTTCTCATTT16910.1085764478357908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.012.4466144
TAAGACG550.003071520712.0892944
CCAACGA1052.7243914E-711.76033419
CTAAGAC1501.7644197E-1011.39847853
CTAGGAC2100.011.3080133
TCCGTAC600.00588150911.0818543
GACAACG700.001493067110.8556937
GTAAACC803.7753384E-410.6860733
TAGGACT2500.010.638584
GTAGAAC4650.010.6222383
GTCCTAA8550.010.5677181
AGGACCT12550.010.444825
GACGTGG8100.010.4368787
ACTGTTC4100.010.4254378
TGGCGAG15350.010.39600718
GGACGTG15450.010.39026
GTAGGAC17950.010.2131213
AGGACGT15450.010.2057585
TTAGGAC10100.010.157063
TGGACTG2250.010.1319815
Produced by FastQC (version 0.11.5)