FastQCFastQC Report
Thu 26 May 2016
SRR1511903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27670.17766471387441346No Hit
CTGTAGGACGTGGAATATGGCAAGA25170.16161260745280037No Hit
GTCCTAAAGTGTGTATTTCTCATTT24830.159429520979461No Hit
GTATCAACGCAGAGTACTTTTTTTT24410.15673276710063No Hit
CTTTAGGACGTGAAATATGGCGAGG22940.14729412852472154No Hit
GTCCTACAGTGTGCATTTCTCATTT17900.11493308197874957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.013.46915151
TAGGACC6850.012.9029164
AGGACCT10450.011.5500255
GTATTAG1201.1924385E-711.1371131
AAAGGCG951.3541347E-511.0043545
GGACGTG12850.010.866076
TCCAACG1252.240049E-710.63976618
CTAGGAC2150.010.6088483
GACGTGG6450.010.598637
CTGTAGG16150.010.5805031
GTAGGAC16000.010.5729333
ACCGTGC909.5691204E-510.5495628
GGACCTG10900.010.5442856
AGGACGT14250.010.5375025
GATATAC4350.010.5336261
AATGTCC4250.010.4999188
GTATCAA15200.010.4881471
TCTAGGA2550.010.4355012
GTCCTAA7750.010.3467371
TGTAGGA16850.010.321592
Produced by FastQC (version 0.11.5)