FastQCFastQC Report
Thu 26 May 2016
SRR1511902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240374
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24150.19469934068272957No Hit
GGTATCAACGCAGAGTACTTTTTTT13990.1127885621594777No Hit
TATCAACGCAGAGTACTTTTTTTTT13610.10972497004935608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5750.013.3971071
GACAGCG550.003070771512.089377
TCTATAC1604.5474735E-1111.2798153
GACGTGA1605.4387783E-1010.686147
TTAGGAC2500.010.6386453
GTATAAA1951.4551915E-1110.2418531
TGGCGAG3450.010.18710818
CTATCCT856.5986626E-410.0575444
GTATCAA12600.09.9631621
GTATAAG1752.5211193E-99.7820151
GTCCTAA2450.09.704381
GGACGTG4350.09.6079726
TATAGGG800.0044742169.5102922
GTCTTAT900.00110220839.5102911
TAGGAGT1502.64934E-79.4987924
CGAAATC900.00111447669.49879113
ATAAGAC1304.241072E-69.4987913
AGGACGT4550.09.3944085
ATGGCGA3950.09.37855217
GGCGAGG1856.697519E-99.24206619
Produced by FastQC (version 0.11.5)