FastQCFastQC Report
Thu 26 May 2016
SRR1511902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240374
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28980.23363920881927547No Hit
GGTATCAACGCAGAGTACTTTTTTT17150.13826474918048912No Hit
TATCAACGCAGAGTACTTTTTTTTT16670.1343949486203355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG400.005262118314.2565985
AACCGTG551.96941E-413.810097
GGTATCA7100.013.038551
CTACACC1354.6875357E-911.2644724
TAGGACG3450.010.7441034
GTAGGAC3150.010.5604433
TAAGACC1106.0075818E-610.3684354
GTGTAGG1502.273191E-810.1799261
GCCTAAG750.002548060110.1799261
CGTGGAA2151.8189894E-1210.1568399
TCTATAC750.002636778910.1380253
ACACCGT750.002647390810.1331196
GACGTGG2450.010.0757297
ATAGTAC856.5629487E-410.063483
GAGTAAG951.5688589E-410.045981
GTATCAA13950.09.9883691
CTAAGAC1151.0199741E-59.9176333
GGACGTG3750.09.879796
TGGCGAG2900.09.82579218
TAATACT1554.02797E-89.8109924
Produced by FastQC (version 0.11.5)