FastQCFastQC Report
Thu 26 May 2016
SRR1511901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047897
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29770.28409280683120575No Hit
GGTATCAACGCAGAGTACTTTTTTT19500.18608699137415224No Hit
TATCAACGCAGAGTACTTTTTTTTT19270.1838921191682007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACCG500.001499886613.29893217
GGTATCA5750.013.230851
GGTCTAG604.0550792E-412.6795641
TGGGCCG701.0915506E-412.2127225
GACTAGG550.003044004612.103221
TGGTATC1900.012.0122192
TATAGGG600.005829161511.0946192
CGTGCGC600.005879097611.08191410
ACACCGG600.005879097611.0819146
GTGGTAT2150.011.057761
GTATCAA13050.011.00353051
GTTACAC700.001492293310.8557533
TCTATAC1053.4674122E-610.8557523
CTGGTCG803.7729272E-410.68613159
TAAGGTT803.7729272E-410.68613155
GTCTTAG1105.9650756E-610.3741881
CAGAACC2151.8189894E-1210.16148954
GTATTAC750.00262408710.1436531
AAGGCGT856.596887E-410.0575356
TTACACC856.596887E-410.0575354
Produced by FastQC (version 0.11.5)