FastQCFastQC Report
Thu 26 May 2016
SRR1511901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047897
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34100.3254136618389021No Hit
TATCAACGCAGAGTACTTTTTTTTT22850.2180557821999681No Hit
GGTATCAACGCAGAGTACTTTTTTT22540.21509747618325084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10930.10430414439587096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.014.2784311
CGCCCTA500.001504749913.29321916
ACCGTCC500.001505291213.2925858
TGGTATC1650.013.2495822
TAGAACC701.084758E-412.2208884
GTGGTAT2150.011.5397871
TACACTA855.28958E-511.1825125
ATACTAG600.005681430511.1329361
TAACCTA600.00584990811.0893245
CTAATTG600.00584990811.0893243
GTCATAA1053.2816697E-610.9057331
CTAGTAT700.001483991910.8630123
GTGCTAC803.616084E-410.7353311
GTCTTAT909.0864065E-510.6027961
GTGTTAC1002.281058E-510.4967671
TATACTG1105.999149E-610.3692395
GTATCAA14550.010.362321
GTACTAT856.324014E-410.1038411
TTGATAC951.6337258E-410.0054053
GTCCTAG1201.6106993E-59.5425161
Produced by FastQC (version 0.11.5)