FastQCFastQC Report
Thu 26 May 2016
SRR1511900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407358
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26750.1900724620174824No Hit
TATCAACGCAGAGTACTTTTTTTTT16820.11951472191155342No Hit
GGTATCAACGCAGAGTACTTTTTTT15940.11326187082462316No Hit
CTGTAGGACGTGGAATATGGCAAGA14770.10494842108404542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT551.9620296E-413.8164516
GGTATCA7150.011.1721431
TAGACAG2050.011.1205585
TAGGACC3100.011.0308764
GTACTAA1053.4230761E-610.8681381
AGGACCG803.7745645E-410.6861615
AGGACGT9250.010.4743635
GGACGTG8900.010.4593646
TAGGACT1659.295036E-1010.3623384
GGCGAGG4150.010.30028119
GACGTGG5100.010.2438147
TGGTATC2600.010.2411312
GTCCTAA5700.010.1769641
TTAGGAC5850.09.9047423
GTCCTAC7800.09.8753771
GTCTTAT1451.5919977E-79.8375391
AAATGTC3200.09.7956487
GTCCTAG1752.5247573E-99.7813241
GTATCAA13550.09.6850761
TTTAGGA6700.09.6515562
Produced by FastQC (version 0.11.5)