FastQCFastQC Report
Thu 26 May 2016
SRR1511900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407358
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31680.2251026391294894No Hit
GGTATCAACGCAGAGTACTTTTTTT19680.13983648794407677No Hit
TATCAACGCAGAGTACTTTTTTTTT19200.13642584189666027No Hit
GTCCTAAAGTGTGTATTTCTCATTT15630.111059161919No Hit
GTCCTACAGTGGACATTTCTAAATT14870.10565897234392385No Hit
CTGTAGGACGTGGAATATGGCAAGA14680.10430892495015481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG456.739064E-414.7843335
GACTATC500.001505269113.2931357
GCGCCAC907.502036E-611.60511813
GGTATCA8400.011.5911251
TCTATAC1251.8186256E-811.4050573
GGACGTG8750.010.963956
CCATCGC700.00149413910.85462419
AGGACGT9300.010.8327615
GTCCTAC8450.010.7317771
TTAGGAC5750.010.5786043
TTAGATA1451.4320904E-810.487414
TAGGACG9800.010.4740324
CGCCACC1106.0800776E-610.35828714
GACGTGG5150.010.3247657
GGCGAGG3900.010.22839619
TGGCGAG7800.010.22803318
ATTAGAC750.002637580710.1378293
CCTAGAC1502.4256224E-810.1378293
GTATATA2650.010.0859521
GACAGCG856.6234777E-410.0536327
Produced by FastQC (version 0.11.5)