FastQCFastQC Report
Thu 26 May 2016
SRR1511899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593271
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21190.13299683481341215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.012.727511
GAAGCGT701.09217945E-412.2127926
ATTACAC1752.0008883E-1110.8558163
TAGGACG4250.010.72814
AGGACGT3850.010.6090925
GTAGGAC4000.010.4487233
TTACACC1106.0531875E-610.3623694
GATCTAA750.002624896410.1438731
GTATTAG1054.0577637E-59.9627321
GGACGTG4200.09.9511646
TAGGACC2300.09.9118324
TTATACC1252.5731697E-69.8787924
GTATCAA13850.09.8188671
GTCATAT2106.548362E-119.5098811
TGTAGGA5100.09.5098812
GTCTTAG2550.09.3234131
GTGGTAT2252.5465852E-119.2985511
GTTCTAG2151.03682396E-109.2887211
GTATAAG1856.5720087E-99.2528571
GTAATAC1356.8549816E-69.147033
Produced by FastQC (version 0.11.5)