FastQCFastQC Report
Thu 26 May 2016
SRR1511899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593271
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24230.15207707916606777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACC1153.7471182E-1013.2228994
GGTATCA7000.012.9498891
TCTACAC1850.011.8157323
GTATCAA15300.010.6022491
CTTATAC1752.3464963E-1010.3185833
GTCTTAA1855.4569682E-1110.31570151
ATTATAC1303.8182407E-710.2350313
GTATTAT2002.3646862E-1110.0191251
GTATAAG2105.456968E-129.9964071
AGGACGT4800.09.7019585
TTAGGAC3350.09.6458083
TAGGACG4750.09.6044
GTGTAGA2900.09.5420251
GTATTAC1106.512685E-59.5420241
GAGTAGT1002.635446E-49.5420241
GGTTTAC1106.512685E-59.5420241
GTAGGAC4900.09.5039593
TAGGACC2303.6379788E-129.5039594
GTTATAC1201.6915592E-59.5039593
TACTCCG1106.803889E-59.5039585
Produced by FastQC (version 0.11.5)