FastQCFastQC Report
Thu 26 May 2016
SRR1511898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400987
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26420.1885813358724956No Hit
GGTATCAACGCAGAGTACTTTTTTT16340.11663206011190681No Hit
TATCAACGCAGAGTACTTTTTTTTT15730.11227798687639501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.013.6068271
CGGTAGC550.003043747712.1038261
GACTGCG855.3277676E-511.1752337
TCTAGAC1106.053253E-610.3621193
GTCCTAC1303.782843E-710.2416981
TTAGTAC951.6459925E-49.9985343
GTATCAA13700.09.9960691
CTTACAC2201.8189894E-129.9303643
CTACCCT1151.0276954E-59.9115914
TCAGTAC1554.0681698E-89.8050153
TAGACAG2151.0913936E-119.7195065
GTCTAAT1002.7250207E-49.5101481
ACTGCGT1002.7574698E-49.4989468
TCTACAC1401.0598033E-69.4986093
TCTATAC1401.0598033E-69.4986093
GTGGTAT2550.09.3236751
GTATTAA2252.5465852E-119.2988121
GTCTTAG1752.6200723E-89.238431
GTATTAT1451.6802296E-69.1822121
GTATAGG1356.750144E-69.1579211
Produced by FastQC (version 0.11.5)