FastQCFastQC Report
Thu 26 May 2016
SRR1511898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400987
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30090.214777153535329No Hit
TATCAACGCAGAGTACTTTTTTTTT18850.1345480008022915No Hit
GGTATCAACGCAGAGTACTTTTTTT18320.1307649535648796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC350.002176875716.27806515
CGCTACC400.00526895914.2539912
GGTATCA7950.013.9292011
ATCATAC853.9302013E-612.2975613
ACGAACA550.003079821412.08480111
CTAGGGT1052.7090573E-711.76524
CCCTAGA1501.9663275E-910.8191461
TTAGAAC1158.7805347E-710.7421383
GTACTAA909.054909E-510.6070071
GTATAAA1951.4551915E-1110.2806371
GCTCTAG951.5648403E-410.0487421
GTATTAT1451.5050682E-79.8754891
TAAGACT1554.0414307E-89.8091994
GTATCAA16850.09.68794251
GTATAAC1409.898449E-79.5463061
TCTAGAC1002.7466618E-49.5026613
TAGGCTG1401.0536587E-69.5026615
TATACCA1201.6938304E-59.5026615
GTATTAC1451.5957976E-69.2171231
TAAGGGA1451.6982976E-69.1749834
Produced by FastQC (version 0.11.5)