FastQCFastQC Report
Thu 26 May 2016
SRR1511897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498522
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24430.1630273029024599No Hit
GGTATCAACGCAGAGTACTTTTTTT15040.10036556019864906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT500.00150117213.2980384
GGTATCA8050.013.2339881
CGTGCGC604.0969686E-412.66479910
ACTGCGC658.033063E-411.6905838
GTCCTAT2000.011.4143141
TGGACCG752.0765644E-411.3983185
GTATAAG1701.0913936E-1111.1905041
GCGCCAC855.330336E-511.17482213
TAGGACC1701.0913936E-1111.1748224
TCCTATA2100.010.8707752
CCTATAC1158.8305933E-710.7375463
CCCTTAT909.3933515E-510.56880951
CTTATAC1451.4457328E-810.4812123
GTATCAA15050.09.9859451
GTCTTAC1151.0108692E-59.925491
GTGCGCC1151.0278603E-59.91158111
ATTACAC1356.4521373E-79.8503993
GTCCACT2800.09.83783411
TAAACTA1451.6194826E-79.8261375
TTAATAC1856.493792E-109.7553173
Produced by FastQC (version 0.11.5)