FastQCFastQC Report
Thu 26 May 2016
SRR1511897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498522
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28660.19125511670832995No Hit
TATCAACGCAGAGTACTTTTTTTTT17850.11911737031555093No Hit
GGTATCAACGCAGAGTACTTTTTTT16900.11277779038279051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7050.012.452061
TGGTATC2600.011.6966242
GTATAAA2050.011.1712011
CTTATAC1900.011.0040613
CTAGGGT1408.29823E-910.8611514
GTATCAA15700.010.5145071
GTGGTAT3200.010.4366371
CGCCCTC750.00265919210.1279414
TAGGACT1803.9108272E-1010.031484
TCTAGGC1356.406699E-79.8554893
GTATTAA1659.498763E-99.8312221
CTAGGAC2151.0913936E-119.7245183
GTCCTAA3150.09.6935291
GTTCTAA1606.1925675E-89.54206851
GTACTAA900.00106935189.54206851
GGGTAAA1409.96055E-79.5420681
GTCTAGG1502.4817018E-79.5420681
GATCTAA1106.511653E-59.5420681
GCATTAG1304.0045634E-69.5420671
TCTAGGA2401.8189894E-129.5035082
Produced by FastQC (version 0.11.5)