FastQCFastQC Report
Thu 26 May 2016
SRR1511896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358984
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22590.16622712261513015No Hit
TATCAACGCAGAGTACTTTTTTTTT13740.10110494310455458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.013.2119831
GGCGTGC701.0919098E-412.2128438
GCGTGCG658.0302387E-411.6909279
GACTTAG855.2663116E-511.1879111
TTAATCA600.005880182611.0820254
AAGGCCG600.005880182611.0820255
AAGGCGT951.3615176E-510.9987016
CTAGACA1303.2690878E-810.9602434
ACCCTAG909.413967E-510.566361
AGGCGTG909.523536E-510.5543097
GTATCAA13750.010.2359221
AACACCG750.002649800410.1321365
TATGCTA951.6454388E-49.9988185
GTCCTGA3250.09.6560281
GGATAAT800.00447688349.5097251
CTAGTAC900.00111452719.49887853
TAGTACA1606.6389475E-89.49887854
CTTAGAC1106.841817E-59.4988783
GTATAAT2151.03682396E-109.28856851
GTCTTAG1752.6215275E-89.2380181
Produced by FastQC (version 0.11.5)