FastQCFastQC Report
Thu 26 May 2016
SRR1511896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358984
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27860.20500609278696438No Hit
GGTATCAACGCAGAGTACTTTTTTT16010.11780859818805814No Hit
TATCAACGCAGAGTACTTTTTTTTT15960.11744067626991929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC350.002177689716.2771058
GTGGTAT2600.012.1186531
GGTATCA6950.011.9522091
ATTACCC657.995974E-411.6968863
TGGACCG752.0659217E-411.4044645
TAATACT1900.011.0043084
TGGTATC2600.010.9658312
TTATACT1158.7672015E-710.7433364
CTTATAC803.756398E-410.6916863
GTATCAA14550.010.1714411
CGTGCTC856.630657E-410.05239610
ATAATAC1151.0210708E-59.9169253
GTAATAC1451.6062586E-79.8314343
CTTAGAC1451.6062586E-79.8314343
GTATAAC900.00106312239.548031
GTACTAC1201.600836E-59.5480291
ATAGTAC900.00110934319.503723
CCAGTAC2151.05501385E-109.2827043
TCTACAC1651.0578515E-79.2157293
ATAAGGG1151.10528745E-49.0905153
Produced by FastQC (version 0.11.5)