FastQCFastQC Report
Thu 26 May 2016
SRR1511895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277354
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34630.2711073046312925No Hit
GTCCTACAGTGGACATTTCTAAATT29690.23243360885079625No Hit
CTGTAGGACGTGGAATATGGCAAGA27550.21568022646815213No Hit
GTCCTAAAGTGTGTATTTCTCATTT24300.19023700555993092No Hit
GGTATCAACGCAGAGTACTTTTTTT20240.15845255113304535No Hit
CTTTAGGACGTGAAATATGGCGAGG20010.15665195396107892No Hit
TATCAACGCAGAGTACTTTTTTTTT18040.1412294477490187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4300.014.800194
CCAACGA851.717126E-814.52845419
TCCAACG1350.014.07314718
GGTATCA6550.012.9227231
CGGTTTC1001.4443322E-712.34821913
GTATAGA2200.011.2397321
AGGACCT8950.011.0375155
GATATAC3550.010.9840071
GTCCTAA7600.010.8870691
GTACTAA1408.198185E-910.8691921
CGGTAGG700.00147735210.8691921
ACGAAAT803.7747106E-410.68595912
AATCCCG1909.094947E-1210.49930819
ACTGTTC3800.010.4984868
GTGTAGA2450.010.4810061
GTAGGAC13350.010.3880153
GTGTTAC1105.9618797E-610.3751371
ACAGTGC2400.010.2901828
GGCGAGG5100.010.24442319
TGGCGAG12650.010.21276618
Produced by FastQC (version 0.11.5)