FastQCFastQC Report
Thu 26 May 2016
SRR1511895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277354
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41370.32387263045326514No Hit
GTCCTACAGTGGACATTTCTAAATT32410.25372762758013834No Hit
CTGTAGGACGTGGAATATGGCAAGA29790.23321647718643385No Hit
GGTATCAACGCAGAGTACTTTTTTT24600.19258561056684365No Hit
GTCCTAAAGTGTGTATTTCTCATTT24120.18882784255578328No Hit
CTTTAGGACGTGAAATATGGCGAGG23750.18593122971392426No Hit
TATCAACGCAGAGTACTTTTTTTTT22150.17340533634372304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCC307.754731E-418.9879449
GGACCGT508.718433E-515.1986856
TGCACCG400.005258165314.2582615
GGTATCA7950.013.204021
CGTTTCC802.010247E-613.05574514
CCGTTTC802.010247E-613.05574513
TAGGACC4350.012.6740084
GGTCTAC1457.2759576E-1212.5044981
AACCCCG550.00305464412.0979175
CCGTGCA951.0472777E-611.9923869
GCCGGTT752.0855216E-411.39276611
CCTACAC1850.011.3038453
TCCAACG1451.2205419E-911.13565818
GTGCAAC600.00590250611.07630111
CCCGTTT951.36915605E-510.9930212
AGCACCG700.00148389510.8634365
GAACAGT2800.010.8562036
CGAAATC700.00149753510.85152813
TCCCGTT1053.4904715E-610.85025411
TGTAGGA16300.010.7884592
Produced by FastQC (version 0.11.5)