FastQCFastQC Report
Thu 26 May 2016
SRR1511894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606597
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28340.1763976902732919No Hit
GGTATCAACGCAGAGTACTTTTTTT16990.10575147345600669No Hit
TATCAACGCAGAGTACTTTTTTTTT16200.10083424779207231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT400.005284199514.2479694
GGTATCA7750.012.2723611
TCGAACT907.4701456E-611.609817519
TAATACT2150.011.4867354
GTAATAC1104.977137E-711.2256743
ATGGGCG700.00149322410.8555965
TTATACC1002.4022862E-510.4485114
AGGACCG1106.054748E-610.362165
GGCGTGC750.002650785710.1318898
TAGGACG3100.010.1114624
GTACCGT856.6018436E-410.057396
ACCGAGC856.6018436E-410.057398
TTACACC856.6018436E-410.057394
GACGTGA1901.0004442E-109.9985757
CTCGAAC1054.1094707E-59.95127218
CCGTGCC1054.110876E-59.9509629
TCAGGAC2201.8189894E-129.9304043
AGAACCG1151.0279471E-59.9116315
GTATCAA15500.09.6951651
GAATTAA1304.1746644E-69.5110791
Produced by FastQC (version 0.11.5)