FastQCFastQC Report
Thu 26 May 2016
SRR1511894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606597
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33360.20764385841626742No Hit
GGTATCAACGCAGAGTACTTTTTTT19940.1241132654922174No Hit
TATCAACGCAGAGTACTTTTTTTTT19600.12199699115584058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG1058.913048E-1114.4679869
CGTGCGC1101.8735591E-1013.8103510
GGTATCA8350.013.7153891
AGGCGTG1251.4224497E-912.1531087
TCGAACT802.8676055E-511.87455319
GTATAAG1751.8189894E-1211.4523491
AAGGCGT1354.7348294E-911.2581346
AACCGTG855.3537107E-511.1701367
GTGCGCC1303.2927346E-810.95532511
GCTATAC1502.4272595E-810.1376873
TTACACG750.002638235710.1376874
GTATCAA17900.010.0234711
CCTAATA1151.0209818E-59.9173022
TAGGACC1356.4026426E-79.8560854
GGCGTGC1451.62976E-79.8220178
GGACTGT2750.09.6717616
TATGGCG2850.09.6617916
ACTGCGT900.0011193459.4946168
GGACGTG2950.09.3380496
GTCCTAC3700.09.2856881
Produced by FastQC (version 0.11.5)