FastQCFastQC Report
Thu 26 May 2016
SRR1511893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381414
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21500.15563762926971927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.012.1801021
GTCCTAA2950.010.9612451
AGGACCG803.7745514E-410.6861365
TCTACGC803.7745514E-410.6861363
GTATCAA12600.010.6426931
CTTAGAC1002.4012239E-510.4486663
GTATTAG1409.524956E-810.1898131
TAGGACT2550.010.057544
GGACCGT856.599697E-410.0575396
GTACTAG951.6253162E-410.0110441
AGCACCG951.6456404E-49.9987235
ATAGGAC1901.0004442E-109.9987233
TTAAGAC951.6456404E-49.9987233
GTAGAAC2107.2759576E-129.9511113
CTAGCAC1651.0228177E-89.786633
TGGTATC1752.5229383E-99.7818642
GTGGTAT2450.09.7045831
GTCCTAC5000.09.7007021
TAGACAG2850.09.6654325
GGATATA900.00110216359.5104921
Produced by FastQC (version 0.11.5)