FastQCFastQC Report
Thu 26 May 2016
SRR1511893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381414
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24500.17735450777247083No Hit
GGTATCAACGCAGAGTACTTTTTTT15220.11017696360395943No Hit
TATCAACGCAGAGTACTTTTTTTTT14190.10272083531801474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA602.4562305E-514.3173031
GGACCGT500.001499894513.2994176
GGTATCA6850.012.2620211
GCGTGCG701.0970943E-412.20667659
TAATACC1103.7856807E-812.0969514
GTAATAC951.0332278E-612.0059973
TGGCGAG4400.011.87490918
GTAGGAC5150.011.8117253
TATGGCG4900.011.04493616
CTAATAC700.001485017510.8625683
TGTAGGA6050.010.8401242
TAGGACG4600.010.7444964
GGCGAGA2850.010.66697219
ACCGTGC909.572679E-510.548988
TGGTATC2350.010.515892
GACGTGG2800.010.5113057
TAGGACA2850.010.3384964
ATATGGC8850.010.19175715
GGACGTG4850.010.185126
GCATTAG750.002545744410.1811931
Produced by FastQC (version 0.11.5)