FastQCFastQC Report
Thu 26 May 2016
SRR1511892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243046
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25130.20216468256202907No Hit
TATCAACGCAGAGTACTTTTTTTTT15540.12501548615256394No Hit
GGTATCAACGCAGAGTACTTTTTTT14790.1189819202185599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTC456.7668443E-414.7760897
CGTGCGC400.005282323314.2483710
GGTATCA5950.013.744451
ATTATAC903.6417987E-813.7206543
GCACCGT655.456624E-513.1523436
GGTTAGA752.0545379E-411.411091
CTAACAC855.3268377E-511.1751933
AAGGCGT600.00587953911.0820676
TCGAACT951.3612007E-510.99874319
AGGACCG700.001492461210.8559025
ACTAAAG700.001492461210.8559023
TTATACT1252.2413042E-710.6387834
CCTCGAA909.521669E-510.55434917
GTATCAA13250.010.4781081
CTCGAAC1002.40034E-510.44880518
ACAATAC1201.524535E-610.290493
TTACACA1856.002665E-1110.2690964
TATACTG1951.4551915E-1110.22965
CAATACT1409.692303E-810.1774074
GTAGGAC2151.8189894E-1210.161633
Produced by FastQC (version 0.11.5)