FastQCFastQC Report
Thu 26 May 2016
SRR1511892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243046
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29790.23965323889864093No Hit
TATCAACGCAGAGTACTTTTTTTTT18360.14770169406441916No Hit
GGTATCAACGCAGAGTACTTTTTTT17610.14166812813041513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG456.792425E-414.7687379
GGTATCA7000.013.9121771
GTACTAG751.9791657E-411.45708751
CCCTAGA1104.6539753E-711.2834961
GCCTAAA600.00566007111.1388351
ACCGTGT600.005901358611.0765518
TAGGACA1752.0008883E-1110.861864
CAGTGCG803.790906E-410.6809629
TAATACA1752.3283064E-1010.3187674
TAGACAG1752.3283064E-1010.3187675
GTATCAA14550.010.3021921
GGGTTAT750.0025394610.1840771
AGTACTA856.5646856E-410.0631945
GTCCTAG1252.408602E-69.9294761
ATTACAC1151.0203254E-59.9173513
TAGACTG1252.5532336E-69.8842945
TAATACT1554.029789E-89.8107134
TTAGGAC1951.6189006E-109.7478233
GTCCTAC2303.6379788E-129.5475731
GTAATAC1201.69025E-59.5041283
Produced by FastQC (version 0.11.5)