FastQCFastQC Report
Thu 26 May 2016
SRR1511891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879404
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15180.17261690872454527No Hit
GGTATCAACGCAGAGTACTTTTTTT9710.11041569062683362No Hit
TATCAACGCAGAGTACTTTTTTTTT9150.10404774142487412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1250.015.9580414
TGTACTA707.26317E-613.5697635
GTACTAA751.460663E-512.6802531
TACTAAG604.0540166E-412.6795312
CTAGTAC604.0931217E-412.66511253
GGTATCA3450.012.1289381
TAATGCT550.003069444312.0894254
CTAGGGT658.025601E-411.6908734
TCTTAGG1001.9028703E-611.4115782
TAGACAG1303.2627213E-810.9601945
CCTACAC1053.4651166E-610.8558113
CTACCCT700.001491813210.8558114
CCTAAAC803.7714397E-410.6861893
GTATTAC909.400625E-510.5668781
GGTCTAC909.400625E-510.5668781
GTATCAA7900.010.4732481
GTCTTAG1105.9576832E-610.3747521
TCTAAAC1106.0432485E-610.3623653
TTTAACC750.00264831110.132093
TAGTACT856.594314E-410.057594
Produced by FastQC (version 0.11.5)