FastQCFastQC Report
Thu 26 May 2016
SRR1511891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879404
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17070.1941087372811586No Hit
GGTATCAACGCAGAGTACTTTTTTT10630.12087732145862425No Hit
TATCAACGCAGAGTACTTTTTTTTT10310.11723849334321881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT250.00605270718.98766314
TCGATGA350.002162127516.2936672
GGCGAGG1550.013.48354819
TTAGACC604.072076E-412.6728524
AAAGGCG751.4694577E-512.6728525
AAGGCGT701.09068365E-412.2132986
GCTGCGT550.003077644212.0851188
TAGGACC1750.011.9486894
GCGCCAC802.8821396E-511.86728913
TAAGCAG657.984561E-411.6980175
GGTATCA4000.011.6924331
CTCGAAC658.0189464E-411.69202818
TCGAACT658.0189464E-411.69202819
TAGGACG3300.011.5207744
TTAGGAC2500.011.4055663
GTCCTAA1451.1223165E-911.1905071
CGTGCGC855.3518277E-511.16921310
TAGACAG1451.202352E-911.1433715
GTGTTAC600.005669884411.1356511
GTAGCAC600.005850914411.0887453
Produced by FastQC (version 0.11.5)