FastQCFastQC Report
Thu 26 May 2016
SRR1511890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152315
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23110.20055280023257527No Hit
TATCAACGCAGAGTACTTTTTTTTT14400.12496582965595346No Hit
GGTATCAACGCAGAGTACTTTTTTT13830.12001926556540529No Hit
GTCCTACAGTGGACATTTCTAAATT12400.10760946442595991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.013.1666581
CCATCGC604.094462E-412.6652519
TAGGACC2050.012.5107964
GGCGTGC1104.9704977E-711.2260168
AGGCGTG1201.2756936E-711.0820937
TGGTATC1855.456968E-1210.79381752
GTCCTAA3650.010.6816261
CTAGACT1355.68798E-810.5543744
GGCGAGG3250.010.52189919
GTGGTAT1909.094947E-1210.5102251
ACTAGAC1002.399857E-510.4488313
TCCAACG1002.399857E-510.44883118
CCTTATA1303.7874815E-710.2402892
GAGTAAG951.6269232E-410.0097391
TAAGGTA951.6448424E-49.998884
CTAGCAC1054.106751E-59.9512673
TAAACTG1151.0267651E-59.9119345
AAAGGCG1252.5705212E-69.8788955
AAGGCGT1252.5705212E-69.8788956
TTCAGGG1651.00444595E-89.7969862
Produced by FastQC (version 0.11.5)