FastQCFastQC Report
Thu 26 May 2016
SRR1511890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152315
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27480.23847645826011116No Hit
GGTATCAACGCAGAGTACTTTTTTT16570.14379748593049643No Hit
TATCAACGCAGAGTACTTTTTTTTT16420.14249575853824692No Hit
GTCCTACAGTGGACATTTCTAAATT12720.11038648286275889No Hit
CTGTAGGACGTGGAATATGGCAAGA12070.10474566416301097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT704.4572153E-714.921411511
CCAACGA851.7131242E-814.5306419
GGACCGG400.00528112714.248736
GGCGTGC1051.378794E-913.564928
TCCAACG1205.0931703E-1113.45947218
CGCCATA500.00149818213.30112418
CGGTTTC751.4851037E-512.66059213
CCGGTTT751.4851037E-512.66059212
CGTGCGC853.9620045E-612.28822110
GCGCCAC853.9620045E-612.28822113
AAGGCGT1103.814239E-812.0898326
GGTATCA7050.011.9046421
CGTTTCC802.8789516E-511.86930514
GTCCTAT1052.582765E-711.8080131
GCGTGCG1052.7342685E-711.7562649
AGGACCG658.0003816E-411.6958355
AGGCGTG1157.1126124E-811.5596717
GTACTAA600.00570545811.1267811
TAATACA1201.2684177E-711.0866784
CCATCGC600.00587039711.08427119
Produced by FastQC (version 0.11.5)