FastQCFastQC Report
Thu 26 May 2016
SRR1511889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745293
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23410.1341322058817631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG550.003071687912.0893419
GGTATCA8000.012.0069861
GTACTAG1353.6379788E-1011.9761651
GACGTGA5600.011.1949767
GTTCGAT600.005881824511.08189611
ACCCCGT600.005881824511.0818966
AGGACGT10800.010.8180415
GGACGTG10700.010.7415976
ATCCCGT803.7756973E-410.68611410
CCAACGA803.7756973E-410.68611419
TTCGATT803.7756973E-410.68611412
TTAGGAC6600.010.6501343
GTATAAG1909.094947E-1210.5115881
TTAGACA1659.313226E-1010.3622924
TAGGACG11400.010.3319934
TAGGACC3500.010.0415544
GTTAGAC1054.1108487E-59.951093
ACGAAAT1054.1108487E-59.9510912
CTTAGAC1151.0279538E-59.9117573
TGGTATC3000.09.82752
Produced by FastQC (version 0.11.5)