FastQCFastQC Report
Thu 26 May 2016
SRR1511889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745293
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26030.14914401192235344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC707.2373186E-613.5762213
ATAACGC500.001495690613.3046983
GGTATCA6350.012.1744081
GTCCTAT1302.4319888E-911.7466281
TAGGACC4000.011.4040264
TCTAGAC1950.011.2090863
GTCCTAA5850.010.9308891
GTCCTAG2800.010.9075821
GTACTAG1407.767085E-910.9075821
TGGACCG909.4816816E-510.5592835
GTCCTAC9100.010.488061
GTATCAA14150.010.4547061
TCCTACA10200.010.34188652
GTATAAA3050.010.0135191
TGGCGAG8250.09.7893718
GGCGAGG3900.09.74535519
GTTTAGG1909.658834E-109.5441351
CTAGATC900.00111006619.5033553
TAGACTG2401.8189894E-129.5033555
CCTACAC2002.6193447E-109.5033553
Produced by FastQC (version 0.11.5)