FastQCFastQC Report
Thu 26 May 2016
SRR1511888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268345
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32240.2541895146825194No Hit
TATCAACGCAGAGTACTTTTTTTTT19290.1520879571409987No Hit
GGTATCAACGCAGAGTACTTTTTTT19180.1512206852236576No Hit
CTGTAGGACGTGGAATATGGCAAGA14760.1163721227268606No Hit
GTCCTACAGTGGACATTTCTAAATT14510.114401050187449No Hit
GTCCTAAAGTGTGTATTTCTCATTT13690.10793593225817896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.012.7270471
TTATACT2150.012.3703994
TATAAGG1553.0559022E-1011.0451172
TTGGACA1053.4696568E-610.8556574
CTATCGG700.001492778810.85565712
CTAATAG700.001492778810.8556573
GTCCTAA4000.010.6999571
AGGACGT9000.010.6596515
GGACGTG8700.010.5905046
TTAGGAC6150.010.5026263
TTTATAC1451.4444595E-810.4813233
GACGTGG4450.010.4592427
GTATCAA14750.010.4460591
GCCCTAA750.00262153910.14514351
GTCTTAA1606.044502E-910.1055141
TGGTATC2650.010.0494352
CCTAATA951.6240767E-410.0116552
TAGGACG10100.09.8748864
GACGTGA4900.09.692557
GTGGTAT3050.09.6669911
Produced by FastQC (version 0.11.5)